ABSTRACT
Peptides corresponding to sequences derived from predicted extra- and intracellular loops of the rat bradykinin receptor were analyzed for interspecies homology as well as for matches within the present dataset of protein sequences to provide a theoretical basis for the specific recognition of the native cognate protein by antibodies raised against these antigens. Apllication of polyclonal antibodies raised against the selected peptides allowed the immunocytochemical localization of the native receptor protein in cells of rat and human origin. The detection of the molecule was achieved by different immunohisto- and immunocytochemical methods in combination with light, fluorescence, confocal optical laser and electron microscopy. These results were compared to localization studies by autoradiography. Distribution and subcellular localization were determined in human neutrophils, human epithelial carcinoma cells (A431) and in rat kidney tissue
Subject(s)
Rats , Humans , Animals , Kinins/physiology , Neutrophils/metabolism , Receptors, Bradykinin/metabolism , Amino Acid Sequence , Autoradiography , Binding Sites, Antibody , Cells, Cultured , Culture Techniques , Species Specificity , Fluorescent Antibody Technique , Immunoenzyme Techniques , Kidney/metabolism , Sequence Homology , Tumor Cells, CulturedABSTRACT
En la Tercera Fase del estudio de la IFCC para la estandarización de la medición de apolipoproteína apo A-I fue investigada la preparación de SP1-01, seleccionada como candidata a material de referencia internacional para apo A-I, según su capacidad para transferir un valor basado en la exactitud a los calibradores de los inmunoensayos, y en producir comparabilidad de los valores en muestras de pacientes. Se asignó un valor de apo A-I de 1,50 g/L al SP1-01, mediante un radioinmunoensayo estandarizado, calibrado con apo A-I purificada, con un valor de masa determinado por análisis de aminoácidos. De acuerdo a un detallado protocolo en común, los participantes transfirieron el valor de masa del SP1-01 al calibrador de cada método. Cada laboratorio analizó 50 muestras de sueros frescos congelados, de individuos donantes, siguiendo un procedimiento similar al adoptado por el Cholesterol Reference Laboratory Network para confirmar que la uniformidad de la calibración asegura la comparabilidad de los valores sobre un amplio rango de valores de apo A-I. El valor medio consensuado sobre cada muestra estuvo en excelente concordancia con el valor asignado por el Northwest Lipid Research Laboratories, con la desviación absoluta promedio entre el valor asignado y el consensuado de 0.01 g/L. El CV interlaboratorios sobre cada una de las 50 muestras tuvo rangos entre 2.1%y 5.6%con un CV%promedio de 3.6%, demostrando que pueden obtenerse resultados de apo A-I comparables por una variedad de métodos inmunoquímicos, mediante el uso de materiales de referencia certificados. Sobre la base de los resultados obtenidos en estos estudios, el SP1-01 ha sido aprobado como material de referencia internacional por la Organización Mundial de la Salud
Subject(s)
Humans , Apolipoproteins A/analysis , Apolipoproteins B/analysis , Clinical Laboratory Techniques/standards , Clinical Trials as Topic , Evaluation Study , Reference Standards , Regression Analysis , Blood Chemical Analysis/standards , Apolipoproteins A/blood , Apolipoproteins B/blood , Calibration/standards , Clinical Laboratory Techniques , Clinical Laboratory Techniques/instrumentation , Blood Specimen Collection/methodsABSTRACT
There is a tendency to interchange dentine adhesives and composite resins when using composite resins for restorations. This study used marginal contraction gaps to test the effect of changing composite resins with a dentine adhesive. Cylindrical butt-joint cavities were prepared entirely in dentine using extracted human teeth. Two groups of control cavities (30 cavities per group) were restored with Prisma-Bond/Prisma-Fil and Heliobond/Heliomolar respectively. Two groups of test cavities (30 cavities per group) were restored using the dentine adhesive. Prisma Universal Bond with Prisma-Fil on one group and Prisma Universal Bond and Heliomolar in the other. Following thermocycling the marginal contraction gaps were measured and assessed. The results showed a significant reduction in marginal contraction gaps when using either composite resin with Prisma Universal Bond. This indicates a compatibility of both resins with the adhesive in this instance.
Subject(s)
Acrylates , Acrylic Resins , Adhesives , Composite Resins , Dental Bonding , Dental Leakage/prevention & control , Dentin , Humans , Polyurethanes , Resin Cements , Resins, SyntheticABSTRACT
The purpose of this study was to test the effectiveness of a universal bonding agent (Prisma Universal Bond) when used in dentine cavities. The measurement of marginal contraction gaps was the basis for determining the effectiveness of this bonding agent. The use of the bonding agent led to a significant reduction in restoration marginal gaps. Thermocycling of restorations with the bonding agent resulted in a widening of the marginal gaps though this was not significant. It was interesting to note that some thermocycled restorations showed no marginal gaps.